Regulation of the intracellular free calcium concentration in acutely dissociated neurones from rat nucleus basalis.
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چکیده
منابع مشابه
Changes in intracellular calcium concentration affect desensitization of GABAA receptors in acutely dissociated P2-P6 rat hippocampal neurons.
The whole cell configuration of the patch-clamp technique was used to study the effects of different cytosolic calcium concentrations [Ca2+]i on desensitization kinetics of gamma-aminobutyric acid (GABA)-activated receptors in acutely dissociated rat hippocampal neurons. Two different intrapipette concentrations of the calcium chelator 1, 2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (...
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The postsynaptic actions of exogenously applied adenosine 5'-triphosphate (ATP) were investigated in nucleus tractus solitarius (NTS) of the rat. Whole cell patch-clamp recordings were used to examine the regulation of voltage-dependent Ca2+ channels (VDCCs) currents (I(Ca)) by ATP in freshly dissociated NTS. Application of ATP inhibited I(Ca) from -905 pA to -741 pA. In addition to this inhibi...
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The hyperpolarization-activated cation current (I(h)), mediated by HCN channels, contributes to intrinsic neuronal properties, synaptic integration and network rhythmicity. Recent studies have implicated HCN channels in neuropathological conditions including epilepsy. While native HCN channels have been studied at the macroscopic level, the biophysical characteristics of individual neuronal HCN...
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A link between the circadian rhythm and the function of Cl(-)-permeable gamma-aminobutyric acid (GABA) type A (GABA(A)) receptors on suprachiasmatic nucleus (SCN) neurons was studied by measuring intracellular activity of Cl(-) (aCl) at different times during a circadian cycle in SCN neurons acutely dissociated from rat brains. To measure aCl, the voltage-clamp mode of the gramicidin-perforated...
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The effects of anoxia on intracellular pH (pH(i)) were examined in acutely isolated adult rat hippocampal CA1 neurons loaded with the H(+)-sensitive fluorophore, 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein. During perfusion with HCO/CO(2)- or HEPES-buffered media (pH 7.35) at 37 degrees C, 5- or 10-min anoxic insults were typified by an intracellular acidification on the induction o...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1993
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1993.sp019628